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Seroprevalence of Avian Reovirus Infection in Apparently Healthy Adult Commercial Layer Flocks*

G. K. Sawale M. Lakshman S. D. Raut N. R. Bulbule M. M. Chawak D. Madhuri Y. N. Reddy
Vol 9(8), 172-176
DOI- http://dx.doi.org/10.5455/ijlr.20190202062036

In order to study the seroprevalence of ARV, a total of 103 sera samples from apparently healthy adult CL flocks unvaccinated against ARV were collected from ten different flocks between December 2016 and August 2018. To study the seroprevalence more closely among the different age group, the birds were categorised into layer/ production group I (21 to 40 weeks), layer/ production group II (40 to 60 weeks) and layer/ production group III (60 weeks and above). Seroprevalence of ARV infection was assessed by ELISA (Idexx Laboratories, USA; Kit no. 99-09264) kit. Out of 103 sera samples, 102 sera samples (99.03%) were positive for ARV antibodies. Age wise seroprevalence of ARV antibodies in different age groups was carried out. All the sera samples were positive in the birds of group 1 (20-40 weeks age) and III (60 weeks and above age) with mean titre of 3640 and 2534, respectively. A total of 97.5 per cent sera samples were positive for ARV in the birds of group II (40 to 60-week age) with mean titre of 3852. The present study indicated widespread prevalence of ARV infection in apparently healthy CL flocks and could be due to nonpathogenic to low pathogenic ARV strain.

Keywords : Avian reovirus Commercial layer Flocks Seroprevalence ELISA

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